{"id":27,"date":"2022-04-30T18:05:54","date_gmt":"2022-04-30T22:05:54","guid":{"rendered":"https:\/\/pressbooks.bccampus.ca\/capalan\/?post_type=chapter&#038;p=27"},"modified":"2022-09-23T15:30:34","modified_gmt":"2022-09-23T19:30:34","slug":"twitch-interpolaiton-techniques","status":"publish","type":"chapter","link":"https:\/\/pressbooks.bccampus.ca\/capalan\/chapter\/twitch-interpolaiton-techniques\/","title":{"raw":"Twitch Interpolaiton Techniques","rendered":"Twitch Interpolaiton Techniques"},"content":{"raw":"Recall that nerves carry information throughout the body in the form of electrical signals called action potentials.\u00a0 These signals are what cause our muscles to contract, our heart to beat, our diaphragm to draw air into our lungs; whether we are consciously aware of it or not, essentially everything that happens in the body happens because the nervous (and endocrine) system tells it to!\r\n\r\nWe have the ability to \u2018tap in\u2019 to this internal electrical messaging system through external methods. By administering an external stimulation to the nerve, we can induce an artificial response in the effector, without involving the central nervous system.\r\n\r\nWhen the effector is a skeletal muscle, we can see the result of this stimulation as a muscle twitch.\u00a0 If the electrical stimulation is low, only a few motor units with be recruited and we will see a small twitch in the muscle.\r\n\r\nAs the amplitude of the stimulation increases, more and more motor units are recruited and the resultant twitch will get larger until a maximum is reached, however the maximum induced by the twitch is well below that which can be achieved through voluntary contraction.\r\n\r\n[caption id=\"attachment_71\" align=\"alignright\" width=\"300\"]<img class=\"wp-image-71 size-medium\" src=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-300x201.jpg\" alt=\"\" width=\"300\" height=\"201\" \/> (Image retrieved from commons wiki)[\/caption]\r\n\r\nScientists primary use muscle twitch to assess Voluntary Activation (VA).\r\n\r\nThis is a way for researchers to know if a human muscle is fully activated (ie-maximal recruitment has occurred) during an attempted maximal voluntary effort (Hales &amp; Gandevia, 1988).\r\n\r\nWhy is this important?\u00a0 Well let\u2019s say you wanted to examine the effect of a fatiguing contraction on the gastrocnemii.\r\n\r\nYou would collect a baseline Maximal Voluntary Contraction (MVC) by having the subject perform plantar flexion against a force plate and record the result.\r\n\r\nThen you would ask them to do submaximal fatiguing plantar flexion contractions, then repeat the MVC.\u00a0 We would hypothesize that the MVC would decrease after the subject was fatigued, but what if the subject wasn\u2019t fatigued and the second time around they just didn\u2019t try as hard during the MVC? We would still see a decrease in the data, but it wouldn\u2019t be due to the intervention!\r\n\r\n[caption id=\"attachment_72\" align=\"alignleft\" width=\"300\"]<img class=\"wp-image-72 size-medium\" src=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-300x251.png\" alt=\"\" width=\"300\" height=\"251\" \/> (Jowko et al., 2019)[\/caption]\r\n\r\nTo prevent this, the researchers will use the twitch interpolation technique to assess voluntary activation.\r\n\r\nEssentially a stimulation is applied to a nerve while the muscle it stimulates is trying to produce an MVC effort and if the force increases with the twitch, the muscle is not fully activated (ie-the person is not producing a maximal recruitment effort).\r\n\r\nIf there is minimal force increase (less than 2%, Hales &amp; Gandevia, 1988) then the muscle is considered fully activated and we can rest assured the person is trying as hard as they can to produce their MVC.\r\n\r\nIf Twitch is used in conjunction with EMG then researchers can also use it to measure:\r\n<ul>\r\n \t<li>Hoffmann\u2019s reflexes \u2013 more commonly called H-reflexes, this is an electrically induced reflex similar to the mechanically induced spinal stretch reflex, with the exception that it bypasses the muscle spindle and therefore gives a better indication of the excitability of the alpha motor neuron, and activity of the mono-synaptic reflex in the spinal cord. This measurement can be used to assess the response of the nervous system to various neurological conditions, musculoskeletal injuries, efficacy of therapy, pain, exercise training and performance (Palmieri, Ingersoll and Hoffman, 2004).<\/li>\r\n \t<li>M-wave \u2013 This is the response at the muscle to the simulation of the nerve (efferent pathway). It is not considered a reflex because the action potential elicited from the stimulation does not pass through the spinal cord.<\/li>\r\n \t<li>F-wave \u2013 This is used primarily to detect demyelinating neuropathies<\/li>\r\n<\/ul>\r\nWhen a muscle twitch is applied, information is collected through two mechanisms: EMG tracings and force tracings.\u00a0 EMG will tell us about recruitment, and force tracings will tell us everything else!\u00a0 Sometime visual inspection can tell you if the muscle is fully activated during contraction, but this will likely not hold up under scientific scrutiny!","rendered":"<p>Recall that nerves carry information throughout the body in the form of electrical signals called action potentials.\u00a0 These signals are what cause our muscles to contract, our heart to beat, our diaphragm to draw air into our lungs; whether we are consciously aware of it or not, essentially everything that happens in the body happens because the nervous (and endocrine) system tells it to!<\/p>\n<p>We have the ability to \u2018tap in\u2019 to this internal electrical messaging system through external methods. By administering an external stimulation to the nerve, we can induce an artificial response in the effector, without involving the central nervous system.<\/p>\n<p>When the effector is a skeletal muscle, we can see the result of this stimulation as a muscle twitch.\u00a0 If the electrical stimulation is low, only a few motor units with be recruited and we will see a small twitch in the muscle.<\/p>\n<p>As the amplitude of the stimulation increases, more and more motor units are recruited and the resultant twitch will get larger until a maximum is reached, however the maximum induced by the twitch is well below that which can be achieved through voluntary contraction.<\/p>\n<figure id=\"attachment_71\" aria-describedby=\"caption-attachment-71\" style=\"width: 300px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-71 size-medium\" src=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-300x201.jpg\" alt=\"\" width=\"300\" height=\"201\" srcset=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-300x201.jpg 300w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-1024x685.jpg 1024w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-768x514.jpg 768w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-65x43.jpg 65w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-225x151.jpg 225w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve-350x234.jpg 350w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-71\" class=\"wp-caption-text\">(Image retrieved from commons wiki)<\/figcaption><\/figure>\n<p>Scientists primary use muscle twitch to assess Voluntary Activation (VA).<\/p>\n<p>This is a way for researchers to know if a human muscle is fully activated (ie-maximal recruitment has occurred) during an attempted maximal voluntary effort (Hales &amp; Gandevia, 1988).<\/p>\n<p>Why is this important?\u00a0 Well let\u2019s say you wanted to examine the effect of a fatiguing contraction on the gastrocnemii.<\/p>\n<p>You would collect a baseline Maximal Voluntary Contraction (MVC) by having the subject perform plantar flexion against a force plate and record the result.<\/p>\n<p>Then you would ask them to do submaximal fatiguing plantar flexion contractions, then repeat the MVC.\u00a0 We would hypothesize that the MVC would decrease after the subject was fatigued, but what if the subject wasn\u2019t fatigued and the second time around they just didn\u2019t try as hard during the MVC? We would still see a decrease in the data, but it wouldn\u2019t be due to the intervention!<\/p>\n<figure id=\"attachment_72\" aria-describedby=\"caption-attachment-72\" style=\"width: 300px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-72 size-medium\" src=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-300x251.png\" alt=\"\" width=\"300\" height=\"251\" srcset=\"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-300x251.png 300w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-65x54.png 65w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-225x188.png 225w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture-350x293.png 350w, https:\/\/pressbooks.bccampus.ca\/capalan\/wp-content\/uploads\/sites\/1419\/2022\/04\/Capture.png 491w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-72\" class=\"wp-caption-text\">(Jowko et al., 2019)<\/figcaption><\/figure>\n<p>To prevent this, the researchers will use the twitch interpolation technique to assess voluntary activation.<\/p>\n<p>Essentially a stimulation is applied to a nerve while the muscle it stimulates is trying to produce an MVC effort and if the force increases with the twitch, the muscle is not fully activated (ie-the person is not producing a maximal recruitment effort).<\/p>\n<p>If there is minimal force increase (less than 2%, Hales &amp; Gandevia, 1988) then the muscle is considered fully activated and we can rest assured the person is trying as hard as they can to produce their MVC.<\/p>\n<p>If Twitch is used in conjunction with EMG then researchers can also use it to measure:<\/p>\n<ul>\n<li>Hoffmann\u2019s reflexes \u2013 more commonly called H-reflexes, this is an electrically induced reflex similar to the mechanically induced spinal stretch reflex, with the exception that it bypasses the muscle spindle and therefore gives a better indication of the excitability of the alpha motor neuron, and activity of the mono-synaptic reflex in the spinal cord. This measurement can be used to assess the response of the nervous system to various neurological conditions, musculoskeletal injuries, efficacy of therapy, pain, exercise training and performance (Palmieri, Ingersoll and Hoffman, 2004).<\/li>\n<li>M-wave \u2013 This is the response at the muscle to the simulation of the nerve (efferent pathway). It is not considered a reflex because the action potential elicited from the stimulation does not pass through the spinal cord.<\/li>\n<li>F-wave \u2013 This is used primarily to detect demyelinating neuropathies<\/li>\n<\/ul>\n<p>When a muscle twitch is applied, information is collected through two mechanisms: EMG tracings and force tracings.\u00a0 EMG will tell us about recruitment, and force tracings will tell us everything else!\u00a0 Sometime visual inspection can tell you if the muscle is fully activated during contraction, but this will likely not hold up under scientific scrutiny!<\/p>\n<div class=\"media-attributions clear\" prefix:cc=\"http:\/\/creativecommons.org\/ns#\" prefix:dc=\"http:\/\/purl.org\/dc\/terms\/\"><h2>Media Attributions<\/h2><ul><li >1200px-Electromyographic_recording_at_adductor_pollicis_muscle_and_stimulation_of_the_ulnar_nerve       <\/li><\/ul><\/div>","protected":false},"author":1360,"menu_order":4,"template":"","meta":{"pb_show_title":"on","pb_short_title":"Twitch Interpolaiton Techniques","pb_subtitle":"","pb_authors":["capalan"],"pb_section_license":"cc-by"},"chapter-type":[],"contributor":[60],"license":[52],"class_list":["post-27","chapter","type-chapter","status-publish","hentry","contributor-capalan","license-cc-by"],"part":3,"_links":{"self":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapters\/27","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/wp\/v2\/users\/1360"}],"version-history":[{"count":2,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapters\/27\/revisions"}],"predecessor-version":[{"id":73,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapters\/27\/revisions\/73"}],"part":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/parts\/3"}],"metadata":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapters\/27\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/wp\/v2\/media?parent=27"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/pressbooks\/v2\/chapter-type?post=27"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/wp\/v2\/contributor?post=27"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/capalan\/wp-json\/wp\/v2\/license?post=27"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}