{"id":83,"date":"2020-08-02T17:12:38","date_gmt":"2020-08-02T21:12:38","guid":{"rendered":"https:\/\/pressbooks.bccampus.ca\/kathleef\/?post_type=chapter&p=83"},"modified":"2025-06-16T16:19:51","modified_gmt":"2025-06-16T20:19:51","slug":"chapter-14-alternative-cloning-methods-2-user-and-golden-gate","status":"publish","type":"chapter","link":"https:\/\/pressbooks.bccampus.ca\/kathleef\/chapter\/chapter-14-alternative-cloning-methods-2-user-and-golden-gate\/","title":{"raw":"Alternative Cloning Methods 2 - Golden Gate Cloning and BioBRICKS","rendered":"Alternative Cloning Methods 2 – Golden Gate Cloning and BioBRICKS"},"content":{"raw":"

Introduction<\/h1>\r\nIn this section we are going to look at the use of restriction enzymes that cut to one side of the recognition sequence rather than inside it. This type of enzyme is called type IIS. This means that the overhang generated will be different for different DNA molecules<\/em>. The value of this is that you can design primers to amplify an insert that has the restriction enzyme sequence, followed by the sequence that will generate your desired overhang.\u00a0 You can do directional cloning easily. You still rely on restriction digest and ligation however, so the lower efficiency of this approach (compared to Gateway) is a potential drawback.\r\n\r\nBIO Bricks is the result of a non-profit organization's attempt to produce a set of components: promoters, coding sequences, and others (like ribosome binding sites) that can be assembled in various combinations to produce novel constructs.\r\n
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Contents<\/h2>\r\n

Learning Outcomes<\/a>\r\nA. Golden Gate Cloning<\/a><\/p>\r\n

A-1. Type IIS Restriction Enzymes<\/a>\r\nA-2. Primer design for Golden Gate Cloning<\/a><\/p>\r\n

B. BioBricks<\/a><\/p>\r\n

B-1. IGEM (this information comes from Labster)<\/a>\r\nB-2. Bio BRICKS<\/a><\/p>\r\n

List of lecture videos (excluding supplemental videos)<\/a><\/p>\r\n\r\n<\/div>\r\n\r\n

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<\/a>Learning Outcomes<\/h2>\r\n<\/header>\r\n
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