{"id":96,"date":"2020-08-06T15:29:34","date_gmt":"2020-08-06T19:29:34","guid":{"rendered":"https:\/\/pressbooks.bccampus.ca\/kathleef\/?post_type=chapter&p=96"},"modified":"2025-06-16T16:17:08","modified_gmt":"2025-06-16T20:17:08","slug":"11-next-generation-sequencing","status":"publish","type":"chapter","link":"https:\/\/pressbooks.bccampus.ca\/kathleef\/chapter\/11-next-generation-sequencing\/","title":{"raw":"Next Generation and nanopore Sequencing","rendered":"Next Generation and nanopore Sequencing"},"content":{"raw":"

Introduction<\/h1>\r\nThe availability of new reagents, tools, and biocomputing power has made it possible to greatly increase the amount of sequence data that can be generated in a relatively short time.\r\n\r\nNext Generation sequencing is a new sequencing approach that does just that. Or it is relatively<\/em> new - it emerged in 2005, which seems more recent to me than it does to you, probably.\u00a0 In this section we'll cover the advantages of Next Gen sequencing, when it is appropriate to use (and when it is not) and how it works. We will also briefly touch upon Nanopore sequencing, a newer approach that is gaining traction in research as it addresses one of the disadvantages of Next Gen sequencing: the short runs.\r\n\r\n \r\n
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Contents<\/h2>\r\n

Learning Outcomes<\/a>\r\nA. The limitations of chain termination sequencing for some types of research<\/a>\r\nB. How Next Generation Sequencing works<\/a><\/p>\r\n

B-1. Preparing the sequences<\/a>\r\nB-2. Generating the clusters<\/a>\r\nB-3. The actual sequencing part<\/a><\/p>\r\n

C. Nanopore sequencing: a very different method<\/a><\/p>\r\n

C-1. How it works in theory<\/a>\r\nC-2. Advantages over other methods<\/a>\r\nC-3. Disadvantages of Nanopore Sequencing<\/a><\/p>\r\n \r\n\r\nList of lecture videos (excluding supplemental videos)<\/a>\r\n\r\n<\/div>\r\n\r\n

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<\/a>Learning Outcomes<\/h2>\r\n<\/header>\r\n
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