Chapter 6. Bone Tissue and the Skeletal System
6.3 Bone Structure — Anatomy and Physiology
6.3 Bone Structure
Learning Objectives
By the end of this section, you will be able to:
- Identify the anatomical features of a bone
- Define and list examples of bone markings
- Describe the histology of bone tissue
- Compare and contrast compact and spongy bone
- Identify the structures that compose compact and spongy bone
- Describe how bones are nourished and innervated
Bone tissue (osseous tissue) differs greatly from other tissues in the body. Bone is hard and many of its functions depend on that characteristic hardness. Later discussions in this chapter will show that bone is also dynamic in that its shape adjusts to accommodate stresses. This section will examine the gross anatomy of bone first and then move on to its histology.
Gross Anatomy of Bone
The structure of a long bone allows for the best visualization of all of the parts of a bone (Figure 1). A long bone has two parts: the diaphysis and the epiphysis. The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The hollow region in the diaphysis is called the medullary cavity, which is filled with yellow marrow. The walls of the diaphysis are composed of dense and hard compact bone.
The wider section at each end of the bone is called the epiphysis (plural = epiphyses), which is filled with spongy bone. Red marrow fills the spaces in the spongy bone. Each epiphysis meets the diaphysis at the metaphysis, the narrow area that contains the epiphyseal plate (growth plate), a layer of hyaline (transparent) cartilage in a growing bone. When the bone stops growing in early adulthood (approximately 18–21 years), the cartilage is replaced by osseous tissue and the epiphyseal plate becomes an epiphyseal line.
The medullary cavity has a delicate membranous lining called the endosteum (end- = “inside”; oste- = “bone”), where bone growth, repair, and remodeling occur. The outer surface of the bone is covered with a fibrous membrane called the periosteum (peri– = “around” or “surrounding”). The periosteum contains blood vessels, nerves, and lymphatic vessels that nourish compact bone. Tendons and ligaments also attach to bones at the periosteum. The periosteum covers the entire outer surface except where the epiphyses meet other bones to form joints (Figure 2). In this region, the epiphyses are covered with articular cartilage, a thin layer of cartilage that reduces friction and acts as a shock absorber.
Flat bones, like those of the cranium, consist of a layer of diploë (spongy bone), lined on either side by a layer of compact bone (Figure 3). The two layers of compact bone and the interior spongy bone work together to protect the internal organs. If the outer layer of a cranial bone fractures, the brain is still protected by the intact inner layer.
Bone Markings
The surface features of bones vary considerably, depending on the function and location in the body. Table 2 describes the bone markings, which are illustrated in (Figure 4). There are three general classes of bone markings: (1) articulations, (2) projections, and (3) holes. As the name implies, an articulation is where two bone surfaces come together (articulus = “joint”). These surfaces tend to conform to one another, such as one being rounded and the other cupped, to facilitate the function of the articulation. A projection is an area of a bone that projects above the surface of the bone. These are the attachment points for tendons and ligaments. In general, their size and shape is an indication of the forces exerted through the attachment to the bone. A hole is an opening or groove in the bone that allows blood vessels and nerves to enter the bone. As with the other markings, their size and shape reflect the size of the vessels and nerves that penetrate the bone at these points.
Bone Markings (Table 2) | ||
---|---|---|
Marking | Description | Example |
Articulations | Where two bones meet | Knee joint |
Head | Prominent rounded surface | Head of femur |
Facet | Flat surface | Vertebrae |
Condyle | Rounded surface | Occipital condyles |
Projections | Raised markings | Spinous process of the vertebrae |
Protuberance | Protruding | Chin |
Process | Prominence feature | Transverse process of vertebra |
Spine | Sharp process | Ischial spine |
Tubercle | Small, rounded process | Tubercle of humerus |
Tuberosity | Rough surface | Deltoid tuberosity |
Line | Slight, elongated ridge | Temporal lines of the parietal bones |
Crest | Ridge | Iliac crest |
Holes | Holes and depressions | Foramen (holes through which blood vessels can pass through) |
Fossa | Elongated basin | Mandibular fossa |
Fovea | Small pit | Fovea capitis on the head of the femur |
Sulcus | Groove | Sigmoid sulcus of the temporal bones |
Canal | Passage in bone | Auditory canal |
Fissure | Slit through bone | Auricular fissure |
Foramen | Hole through bone | Foramen magnum in the occipital bone |
Meatus | Opening into canal | External auditory meatus |
Sinus | Air-filled space in bone | Nasal sinus |
Bone Cells and Tissue
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide a surface for inorganic salt crystals to adhere. These salt crystals form when calcium phosphate and calcium carbonate combine to create hydroxyapatite, which incorporates other inorganic salts like magnesium hydroxide, fluoride, and sulfate as it crystallizes, or calcifies, on the collagen fibers. The hydroxyapatite crystals give bones their hardness and strength, while the collagen fibers give them flexibility so that they are not brittle.
Although bone cells compose a small amount of the bone volume, they are crucial to the function of bones. Four types of cells are found within bone tissue: osteoblasts, osteocytes, osteogenic cells, and osteoclasts (Figure 5).
The osteoblast is the bone cell responsible for forming new bone and is found in the growing portions of bone, including the periosteum and endosteum. Osteoblasts, which do not divide, synthesize and secrete the collagen matrix and calcium salts. As the secreted matrix surrounding the osteoblast calcifies, the osteoblast become trapped within it; as a result, it changes in structure and becomes an osteocyte, the primary cell of mature bone and the most common type of bone cell. Each osteocyte is located in a space called a lacuna and is surrounded by bone tissue. Osteocytes maintain the mineral concentration of the matrix via the secretion of enzymes. Like osteoblasts, osteocytes lack mitotic activity. They can communicate with each other and receive nutrients via long cytoplasmic processes that extend through canaliculi (singular = canaliculus), channels within the bone matrix.
If osteoblasts and osteocytes are incapable of mitosis, then how are they replenished when old ones die? The answer lies in the properties of a third category of bone cells—the osteogenic cell. These osteogenic cells are undifferentiated with high mitotic activity and they are the only bone cells that divide. Immature osteogenic cells are found in the deep layers of the periosteum and the marrow. They differentiate and develop into osteoblasts.
The dynamic nature of bone means that new tissue is constantly formed, and old, injured, or unnecessary bone is dissolved for repair or for calcium release. The cell responsible for bone resorption, or breakdown, is the osteoclast. They are found on bone surfaces, are multinucleated, and originate from monocytes and macrophages, two types of white blood cells, not from osteogenic cells. Osteoclasts are continually breaking down old bone while osteoblasts are continually forming new bone. The ongoing balance between osteoblasts and osteoclasts is responsible for the constant but subtle reshaping of bone. Table 3 reviews the bone cells, their functions, and locations.
Bone Cells (Table 3) | ||
---|---|---|
Cell type | Function | Location |
Osteogenic cells | Develop into osteoblasts | Deep layers of the periosteum and the marrow |
Osteoblasts | Bone formation | Growing portions of bone, including periosteum and endosteum |
Osteocytes | Maintain mineral concentration of matrix | Entrapped in matrix |
Osteoclasts | Bone resorption | Bone surfaces and at sites of old, injured, or unneeded bone |
Compact and Spongy Bone
The differences between compact and spongy bone are best explored via their histology. Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone’s overall function. Compact bone is dense so that it can withstand compressive forces, while spongy (cancellous) bone has open spaces and supports shifts in weight distribution.
Compact Bone
Compact bone is the denser, stronger of the two types of bone tissue (Figure 6). It can be found under the periosteum and in the diaphyses of long bones, where it provides support and protection.
The microscopic structural unit of compact bone is called an osteon, or Haversian system. Each osteon is composed of concentric rings of calcified matrix called lamellae (singular = lamella). Running down the center of each osteon is the central canal, or Haversian canal, which contains blood vessels, nerves, and lymphatic vessels. These vessels and nerves branch off at right angles through a perforating canal, also known as Volkmann’s canals, to extend to the periosteum and endosteum.
The osteocytes are located inside spaces called lacunae (singular = lacuna), found at the borders of adjacent lamellae. As described earlier, canaliculi connect with the canaliculi of other lacunae and eventually with the central canal. This system allows nutrients to be transported to the osteocytes and wastes to be removed from them.
Spongy (Cancellous) Bone
Like compact bone, spongy bone, also known as cancellous bone, contains osteocytes housed in lacunae, but they are not arranged in concentric circles. Instead, the lacunae and osteocytes are found in a lattice-like network of matrix spikes called trabeculae (singular = trabecula) (Figure 7). The trabeculae may appear to be a random network, but each trabecula forms along lines of stress to provide strength to the bone. The spaces of the trabeculated network provide balance to the dense and heavy compact bone by making bones lighter so that muscles can move them more easily. In addition, the spaces in some spongy bones contain red marrow, protected by the trabeculae, where hematopoiesis occurs.
Skeletal System: Paget’s Disease
Paget’s disease usually occurs in adults over age 40. It is a disorder of the bone remodeling process that begins with overactive osteoclasts. This means more bone is resorbed than is laid down. The osteoblasts try to compensate but the new bone they lay down is weak and brittle and therefore prone to fracture.
While some people with Paget’s disease have no symptoms, others experience pain, bone fractures, and bone deformities (Figure 8). Bones of the pelvis, skull, spine, and legs are the most commonly affected. When occurring in the skull, Paget’s disease can cause headaches and hearing loss.
What causes the osteoclasts to become overactive? The answer is still unknown, but hereditary factors seem to play a role. Some scientists believe Paget’s disease is due to an as-yet-unidentified virus.
Paget’s disease is diagnosed via imaging studies and lab tests. X-rays may show bone deformities or areas of bone resorption. Bone scans are also useful. In these studies, a dye containing a radioactive ion is injected into the body. Areas of bone resorption have an affinity for the ion, so they will light up on the scan if the ions are absorbed. In addition, blood levels of an enzyme called alkaline phosphatase are typically elevated in people with Paget’s disease.
Bisphosphonates, drugs that decrease the activity of osteoclasts, are often used in the treatment of Paget’s disease. However, in a small percentage of cases, bisphosphonates themselves have been linked to an increased risk of fractures because the old bone that is left after bisphosphonates are administered becomes worn out and brittle. Still, most doctors feel that the benefits of bisphosphonates more than outweigh the risk; the medical professional has to weigh the benefits and risks on a case-by-case basis. Bisphosphonate treatment can reduce the overall risk of deformities or fractures, which in turn reduces the risk of surgical repair and its associated risks and complications.
Blood and Nerve Supply
The spongy bone and medullary cavity receive nourishment from arteries that pass through the compact bone. The arteries enter through the nutrient foramen (plural = foramina), small openings in the diaphysis ([link]). The osteocytes in spongy bone are nourished by blood vessels of the periosteum that penetrate spongy bone and blood that circulates in the marrow cavities. As the blood passes through the marrow cavities, it is collected by veins, which then pass out of the bone through the foramina.
In addition to the blood vessels, nerves follow the same paths into the bone where they tend to concentrate in the more metabolically active regions of the bone. The nerves sense pain, and it appears the nerves also play roles in regulating blood supplies and in bone growth, hence their concentrations in metabolically active sites of the bone.
Watch this video to see the microscopic features of a bone.
Chapter Review
A hollow medullary cavity filled with yellow marrow runs the length of the diaphysis of a long bone. The walls of the diaphysis are compact bone. The epiphyses, which are wider sections at each end of a long bone, are filled with spongy bone and red marrow. The epiphyseal plate, a layer of hyaline cartilage, is replaced by osseous tissue as the organ grows in length. The medullary cavity has a delicate membranous lining called the endosteum. The outer surface of bone, except in regions covered with articular cartilage, is covered with a fibrous membrane called the periosteum. Flat bones consist of two layers of compact bone surrounding a layer of spongy bone. Bone markings depend on the function and location of bones. Articulations are places where two bones meet. Projections stick out from the surface of the bone and provide attachment points for tendons and ligaments. Holes are openings or depressions in the bones.
Bone matrix consists of collagen fibers and organic ground substance, primarily hydroxyapatite formed from calcium salts. Osteogenic cells develop into osteoblasts. Osteoblasts are cells that make new bone. They become osteocytes, the cells of mature bone, when they get trapped in the matrix. Osteoclasts engage in bone resorption. Compact bone is dense and composed of osteons, while spongy bone is less dense and made up of trabeculae. Blood vessels and nerves enter the bone through the nutrient foramina to nourish and innervate bones.
Review Questions
1. Which of the following occurs in the spongy bone of the epiphysis?
- bone growth
- bone remodeling
- hematopoiesis
- shock absorption
2. The diaphysis contains ________.
- the metaphysis
- fat stores
- spongy bone
- compact bone
3. The fibrous membrane covering the outer surface of the bone is the ________.
- periosteum
- epiphysis
- endosteum
- diaphysis
4. Which of the following are incapable of undergoing mitosis?
- osteoblasts and osteoclasts
- osteocytes and osteoclasts
- osteoblasts and osteocytes
- osteogenic cells and osteoclasts
5. Which cells do not originate from osteogenic cells?
- osteoblasts
- osteoclasts
- osteocytes
- osteoprogenitor cells
6. Which of the following are found in compact bone and cancellous bone?
- Haversian systems
- Haversian canals
- lamellae
- lacunae
7. Which of the following are only found in cancellous bone?
- canaliculi
- Volkmann’s canals
- trabeculae
- calcium salts
8. The area of a bone where the nutrient foramen passes forms what kind of bone marking?
- a hole
- a facet
- a canal
- a fissure
Critical Thinking Questions
1. If the articular cartilage at the end of one of your long bones were to degenerate, what symptoms do you think you would experience? Why?
2. In what ways is the structural makeup of compact and spongy bone well suited to their respective functions?
Glossary
- articular cartilage
- thin layer of cartilage covering an epiphysis; reduces friction and acts as a shock absorber
- articulation
- where two bone surfaces meet
- canaliculi
- (singular = canaliculus) channels within the bone matrix that house one of an osteocyte’s many cytoplasmic extensions that it uses to communicate and receive nutrients
- central canal
- longitudinal channel in the center of each osteon; contains blood vessels, nerves, and lymphatic vessels; also known as the Haversian canal
- compact bone
- dense osseous tissue that can withstand compressive forces
- diaphysis
- tubular shaft that runs between the proximal and distal ends of a long bone
- diploë
- layer of spongy bone, that is sandwiched between two the layers of compact bone found in flat bones
- endosteum
- delicate membranous lining of a bone’s medullary cavity
- epiphyseal plate
- (also, growth plate) sheet of hyaline cartilage in the metaphysis of an immature bone; replaced by bone tissue as the organ grows in length
- epiphysis
- wide section at each end of a long bone; filled with spongy bone and red marrow
- hole
- opening or depression in a bone
- lacunae
- (singular = lacuna) spaces in a bone that house an osteocyte
- medullary cavity
- hollow region of the diaphysis; filled with yellow marrow
- nutrient foramen
- small opening in the middle of the external surface of the diaphysis, through which an artery enters the bone to provide nourishment
- osteoblast
- cell responsible for forming new bone
- osteoclast
- cell responsible for resorbing bone
- osteocyte
- primary cell in mature bone; responsible for maintaining the matrix
- osteogenic cell
- undifferentiated cell with high mitotic activity; the only bone cells that divide; they differentiate and develop into osteoblasts
- osteon
- (also, Haversian system) basic structural unit of compact bone; made of concentric layers of calcified matrix
- perforating canal
- (also, Volkmann’s canal) channel that branches off from the central canal and houses vessels and nerves that extend to the periosteum and endosteum
- periosteum
- fibrous membrane covering the outer surface of bone and continuous with ligaments
- projection
- bone markings where part of the surface sticks out above the rest of the surface, where tendons and ligaments attach
- spongy bone
- (also, cancellous bone) trabeculated osseous tissue that supports shifts in weight distribution
- trabeculae
- (singular = trabecula) spikes or sections of the lattice-like matrix in spongy bone
Solutions
Answers for Review Questions
- C
- B
- A
- C
- D
- C
- C
- A
Answers for Critical Thinking Questions
- If the articular cartilage at the end of one of your long bones were to deteriorate, which is actually what happens in osteoarthritis, you would experience joint pain at the end of that bone and limitation of motion at that joint because there would be no cartilage to reduce friction between adjacent bones and there would be no cartilage to act as a shock absorber.
- The densely packed concentric rings of matrix in compact bone are ideal for resisting compressive forces, which is the function of compact bone. The open spaces of the trabeculated network of spongy bone allow spongy bone to support shifts in weight distribution, which is the function of spongy bone.
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