{"id":903,"date":"2021-08-03T00:41:05","date_gmt":"2021-08-03T04:41:05","guid":{"rendered":"https:\/\/pressbooks.bccampus.ca\/pathology\/?post_type=chapter&#038;p=903"},"modified":"2025-11-05T22:49:35","modified_gmt":"2025-11-06T03:49:35","slug":"a-demonstrative-videolesson-on-histology","status":"publish","type":"chapter","link":"https:\/\/pressbooks.bccampus.ca\/pathology\/chapter\/a-demonstrative-videolesson-on-histology\/","title":{"raw":"Video Lessons on Histology","rendered":"Video Lessons on Histology"},"content":{"raw":"<div class=\"textbox textbox--learning-objectives\"><header class=\"textbox__header\">\r\n<p class=\"textbox__title\">Learning Objectives<\/p>\r\n\r\n<\/header>\r\n<div class=\"textbox__content\">\r\n\r\nBy the end of this section, you will be able to:\r\n<ul>\r\n \t<li>Explain how hematoxylin &amp; eosin staining works and which aspects of the cell it allows to be made visible microscopically.<\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n&nbsp;\r\n\r\nNow that we know what to expect from epithelial tissue, we are ready to look at it microscopically.\r\n\r\nRemember that tissue is normally clear, so we use stains to help distinguish its various components.\r\n<h2>Hematoxylin and Eosin (H&amp;E) Stain<\/h2>\r\nH&amp;E is the most commonly used stain and involves a sequential applications of two different dyes.\r\n\r\n[pb_glossary id=\"393\"]Hematoxylin[\/pb_glossary] is a blue basic dye and stains acidic elements in the tissue such as the nucleic acids (RNA and DNA). Nuclei of cells, therefore, appear as blue circles.\r\n\r\n[pb_glossary id=\"394\"]Eosin[\/pb_glossary] is a pink acidic dye and binds to basic molecules, which are mainly proteins. These are usually found in the cytosol of the cells and in the extracellular spaces. Collagen, an extra-cellular matrix protein, stains pink as do muscle cells.\r\n\r\nPurple staining in an H&amp;E slide indicates the presence of both acidic and basic components. The keratin layer in skin often stains purple due to the combination of pink-staining keratin and dark blue keratohyalin granules.\r\n<h2>Video Demonstration of Looking at the Histology of Epithelial Tissue<\/h2>\r\nHow do we look at tissues like a histologist?\u00a0 This video demonstrates a histological description with H&amp;E staining of thick skin.\r\n\r\n[h5p id=\"44\"]\r\n\r\nNow let's listen to a histopathologist describe lung epithelial tissue using these skills.\r\n<p style=\"text-align: center;background-color: #f0f0f0;padding: 5px\"><sup>Introduction to Histology Staining by Dr. Jonathan Bush, licensed under <a href=\"https:\/\/choosealicense.com\/no-license\/\" rel=\"license\">All Rights Reserved<\/a><\/sup><\/p>\r\nLike riding a bike, interpreting histology is a skill that needs practice.\u00a0 Consider referring to\u00a0<a href=\"https:\/\/pressbooks.bccampus.ca\/pathology\/part\/appendices-introduction-to-histology-for-first-time-learners\/\">Appendices: Introduction to Histology for first-time learners<\/a>\u00a0 \u00a0for a primer on histology of epithelial tissue.\r\n<h1>Section Review<\/h1>\r\n<ul>\r\n \t<li>Hematoxylin &amp; Eosin (H&amp;E) is the most common stain to visualize tissues.\r\n<ul>\r\n \t<li>Blue = DNA\/RNA within nuclei (usually, one nuclei\/cell)<\/li>\r\n \t<li>Pink = protein (e.g. protein in the cytosol or other intracellular fluid)<\/li>\r\n \t<li>White = nothing to stain (e.g. air) OR stain can't bind (e.g. fat)<\/li>\r\n<\/ul>\r\n<\/li>\r\n \t<li>Nuclei are often the biggest structure within the cell, and may appear in either an oval or round shape.<\/li>\r\n<\/ul>\r\n&nbsp;","rendered":"<div class=\"textbox textbox--learning-objectives\">\n<header class=\"textbox__header\">\n<p class=\"textbox__title\">Learning Objectives<\/p>\n<\/header>\n<div class=\"textbox__content\">\n<p>By the end of this section, you will be able to:<\/p>\n<ul>\n<li>Explain how hematoxylin &amp; eosin staining works and which aspects of the cell it allows to be made visible microscopically.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<p>Now that we know what to expect from epithelial tissue, we are ready to look at it microscopically.<\/p>\n<p>Remember that tissue is normally clear, so we use stains to help distinguish its various components.<\/p>\n<h2>Hematoxylin and Eosin (H&amp;E) Stain<\/h2>\n<p>H&amp;E is the most commonly used stain and involves a sequential applications of two different dyes.<\/p>\n<p><a class=\"glossary-term\" aria-haspopup=\"dialog\" aria-describedby=\"definition\" href=\"#term_903_393\">Hematoxylin<\/a> is a blue basic dye and stains acidic elements in the tissue such as the nucleic acids (RNA and DNA). Nuclei of cells, therefore, appear as blue circles.<\/p>\n<p><a class=\"glossary-term\" aria-haspopup=\"dialog\" aria-describedby=\"definition\" href=\"#term_903_394\">Eosin<\/a> is a pink acidic dye and binds to basic molecules, which are mainly proteins. These are usually found in the cytosol of the cells and in the extracellular spaces. Collagen, an extra-cellular matrix protein, stains pink as do muscle cells.<\/p>\n<p>Purple staining in an H&amp;E slide indicates the presence of both acidic and basic components. The keratin layer in skin often stains purple due to the combination of pink-staining keratin and dark blue keratohyalin granules.<\/p>\n<h2>Video Demonstration of Looking at the Histology of Epithelial Tissue<\/h2>\n<p>How do we look at tissues like a histologist?\u00a0 This video demonstrates a histological description with H&amp;E staining of thick skin.<\/p>\n<div id=\"h5p-44\">\n<div class=\"h5p-iframe-wrapper\"><iframe id=\"h5p-iframe-44\" class=\"h5p-iframe\" data-content-id=\"44\" style=\"height:1px\" src=\"about:blank\" frameBorder=\"0\" scrolling=\"no\" title=\"Introduction to Histology\"><\/iframe><\/div>\n<\/div>\n<p>Now let&#8217;s listen to a histopathologist describe lung epithelial tissue using these skills.<\/p>\n<p style=\"text-align: center;background-color: #f0f0f0;padding: 5px\"><sup>Introduction to Histology Staining by Dr. Jonathan Bush, licensed under <a href=\"https:\/\/choosealicense.com\/no-license\/\" rel=\"license\">All Rights Reserved<\/a><\/sup><\/p>\n<p>Like riding a bike, interpreting histology is a skill that needs practice.\u00a0 Consider referring to\u00a0<a href=\"https:\/\/pressbooks.bccampus.ca\/pathology\/part\/appendices-introduction-to-histology-for-first-time-learners\/\">Appendices: Introduction to Histology for first-time learners<\/a>\u00a0 \u00a0for a primer on histology of epithelial tissue.<\/p>\n<h1>Section Review<\/h1>\n<ul>\n<li>Hematoxylin &amp; Eosin (H&amp;E) is the most common stain to visualize tissues.\n<ul>\n<li>Blue = DNA\/RNA within nuclei (usually, one nuclei\/cell)<\/li>\n<li>Pink = protein (e.g. protein in the cytosol or other intracellular fluid)<\/li>\n<li>White = nothing to stain (e.g. air) OR stain can&#8217;t bind (e.g. fat)<\/li>\n<\/ul>\n<\/li>\n<li>Nuclei are often the biggest structure within the cell, and may appear in either an oval or round shape.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<div class=\"glossary\"><span class=\"screen-reader-text\" id=\"definition\">definition<\/span><template id=\"term_903_393\"><div class=\"glossary__definition\" role=\"dialog\" data-id=\"term_903_393\"><div tabindex=\"-1\"><p>A blue basic dye that is used to stain tissues. It turns acidic elements such as RNA and DNA in the tissue blue. <\/p>\n<\/div><button><span aria-hidden=\"true\">&times;<\/span><span class=\"screen-reader-text\">Close definition<\/span><\/button><\/div><\/template><template id=\"term_903_394\"><div class=\"glossary__definition\" role=\"dialog\" data-id=\"term_903_394\"><div tabindex=\"-1\"><p>Eosin is a pink acidic dye that stains basic elements such as most proteins in the tissue pink or red<\/p>\n<\/div><button><span aria-hidden=\"true\">&times;<\/span><span class=\"screen-reader-text\">Close definition<\/span><\/button><\/div><\/template><\/div>","protected":false},"author":1232,"menu_order":7,"template":"","meta":{"pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":["jen-2","lyz-boyd","jonathan-bush"],"pb_section_license":""},"chapter-type":[],"contributor":[59,65,64],"license":[],"class_list":["post-903","chapter","type-chapter","status-publish","hentry","contributor-jen-2","contributor-jonathan-bush","contributor-lyz-boyd"],"part":406,"_links":{"self":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapters\/903","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/wp\/v2\/users\/1232"}],"version-history":[{"count":25,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapters\/903\/revisions"}],"predecessor-version":[{"id":9793,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapters\/903\/revisions\/9793"}],"part":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/parts\/406"}],"metadata":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapters\/903\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/wp\/v2\/media?parent=903"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/pressbooks\/v2\/chapter-type?post=903"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/wp\/v2\/contributor?post=903"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/pressbooks.bccampus.ca\/pathology\/wp-json\/wp\/v2\/license?post=903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}